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- Title
Protective MCMV immunity by vaccination of the salivary gland via Wharton's duct: replicationdeficient recombinant adenovirus expressing individual MCMV genes elicits protection similar to that of MCMV.
- Authors
Guangliang Liu; Fangfang Zhang; Ruixue Wang; Lucille London; London, Steven D.
- Abstract
Salivary glands, a major component of the mucosal immune system, confer antigen-specific immunity to mucosally acquired pathogens. We investigated whether a physiological route of inoculation and a subunit vaccine approach elicited MCMV-specific and protective immunity. Mice were inoculated by retrograde perfusion of the submandibular salivary glands via Wharton's duct with tcMCMV or MCMV proteins focused to the salivary gland via replication-deficient adenovirus expressing individual MCMV genes (gB, gH, IE1; controls: saline and replication deficient adenovirus without MCMV inserts). Mice were evaluated for MCMV-specific antibodies, T-cell responses, germinal center formation, and protection against a lethal MCMV challenge. Retrograde perfusion with tcMCMV or adenovirus expressed MCMV proteins induced a 2- to 6-fold increase in systemic and mucosal MCMVspecific antibodies, a 3- to 6-fold increase in GC marker expression, and protection against a lethal systemic challenge, as evidenced by up to 80% increased survival, decreased splenic pathology, and decreased viral titers from 10(> pfu to undetectable levels. Thus, a focused salivary gland immunization via a physiological route with a protein antigen induced systemic and mucosal protective immune responses. Therefore, salivary gland immunization can serve as an alternative mucosal route for administering vaccines, which is directly applicable for use in humans.--Liu, G., Zhang, F., Wang, R., London, L., London, S. D. Protective MCMV immunity by vaccination of the salivary gland via Wharton's duct: replication-deficient recombinant adenovirus expressing individual MCMV genes elicits protection similar to that of MCMV.
- Subjects
CYTOMEGALOVIRUSES; SALIVARY glands; ADENOVIRUSES; IMMUNE system; SUBMANDIBULAR gland; RECOMBINANT proteins; LABORATORY mice
- Publication
FASEB Journal, 2014, Vol 28, Issue 4, p1698
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.13-244178