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- Title
Production of endothelin 1 by cultured bovine retinal endothelial cells and presence of endothelin receptors on associated pericytes.
- Authors
Takahashi, Kazuhiro; Brooks, Roger A.; Kanse, Sandip M.; Ghatei, Mohammad A.; Kohner, Eva M.; Bloom, Stephen R.; Takahashi, K; Brooks, R A; Kanse, S M; Ghatei, M A; Kohner, E M; Bloom, S R
- Abstract
Endothelinlike immunoreactivity was detected by radioimmunoassay in medium conditioned by cultured endothelial cells obtained from bovine retinal microvessels (9.2 +/- 6.5 pM, n = 4). Sephadex G-25 column chromatography and fast-protein liquid chromatography revealed that most of the endothelinlike immunoreactivity was eluted in an identical position to synthetic endothelin 1. Retinal capillary pericyte-conditioned medium contained 2.9 pM endothelinlike immunoreactivity. In contrast to endothelial cells, retinal pericytes were found to bind endothelin. The dissociation constant and binding capacity were 0.14 nM and 1.5 x 10(5) sites/cell (n = 3), respectively. These findings suggest that endothelin produced by the retinal endothelial cells binds to the pericytes, adding support to the suggestion that pericytes in the retina may have a musclelike function.
- Subjects
PEPTIDE analysis; ANIMALS; BINDING sites; CAPILLARIES; CATTLE; CELL receptors; ENDOTHELINS; ENDOTHELIUM; HIGH performance liquid chromatography; IMMUNOHISTOCHEMISTRY; METABOLISM; RADIOIMMUNOASSAY; RETINA
- Publication
Diabetes, 1989, Vol 38, Issue 9, p1200
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.38.9.1200