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- Title
Characteristics of insulin receptors and insulin action in human myelogenous leukemia cell line K-562.
- Authors
Yamanouchi, Toshikazu; Tsushima, Toshio; Akanuma, Yasuo; Kasuga, Masato; Mizoguchi, Hideaki; Takaku, Fumimaro; Yamanouchi, T; Tsushima, T; Akanuma, Y; Kasuga, M; Mizoguchi, H; Takaku, F
- Abstract
Specific binding sites for insulin have been identified and characterized for the human erythroleukemia cell line K-562. The binding of [125I]-insulin to the cells increased as a function of time, reaching a maximum at 20 min when incubation was performed at 37 degrees C. The binding of [125I]-insulin was dose-dependently inhibited by insulin or proinsulin. Scatchard plot of the binding data was curvilinear, and the number of insulin receptors was approximately 39,000. Insulin at concentrations of 0.05-10.0 ng/ml stimulated CO2 production and DNA and protein synthesis in K-562 cells in a dose-dependent manner, indicating that the insulin binding sites are functionally important in mediating these biochemical events induced by insulin. Maximal insulin responses were elicited at concentrations of less than 5 ng/ml, when (at most) 10% of the insulin receptors were occupied. After binding to the cells, [125I]-insulin was degraded in a time- and temperature-dependent manner. As reported for other types of cells, unlabeled insulin also downregulated insulin receptors in K-562 cells. When the cells were incubated with 1 X 10(-7) M unlabeled insulin for 24 h, the number of insulin receptors decreased by 50% without a change of affinity. K-562 cells may be useful in studying the role of insulin receptors in cell functions induced by insulin.
- Publication
Diabetes, 1985, Vol 34, Issue 4, p347
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.34.4.347