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- Title
Purification, compositional analysis and antioxidant properties of polysaccharides from black ginseng.
- Authors
Li-Hong Gong; Tao Lei; Zhao-Li Zhang; Qi-Chao Liang; Feng-Guo Zhai; Yi-Yan Wu; Xiu-Ping Zhang; Jia-Qi Liu; Jia-Wei Liu
- Abstract
Purpose: To extract, purify black ginseng polysaccharides, and study their compositional analysis and antioxidant properties. Methods: Crude polysaccharides from black ginseng were prepared by hot water extraction and subjected to chromatographic purification on Sephadex G-75 and DEAE-cellulose and Sephadex G-75 columns to yield 4 polysaccharide components: BGP-60, BGP-65, BGP-70 and BGP-80. The BGPs were characterized by chemical analysis, gel permeation chromatography (GPC), gas chromatographymass spectrometry (GC-MS), and Fourier transform-infrared spectroscopy (FT-IR). Finally, the in vitro antioxidant activities of the BGPs were determined through their capacities to scavenge superoxide anion, as well as 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals. Results: The four fractions designated BGP-60, BGP-65, BGP-70 and BGP-80 were polysaccharides with glucose as the main component. They were acidic in nature, with estimated molecular weights (MWs) of 28.6, 26.7, 11.4 and 3.05 kDa, respectively. Fractions BGP-60, BGP-65 and BGP-80 had a- type glycosidic linkage, whereas BGP-70 had ß-type glycosidic linkage. Compared with vitamin C (vit C), it was found that BGP-60, BGP-65, BGP-70 and BGP-80 had strong potential antioxidant activities; BGP-60 exhibited a stronger antioxidant activity than BGP-65, BGP-70 or BGP-80 against DPPH and superoxide anion radicals, while BGP-65 had the highest antioxidant activity against hydroxyl radical. Conclusion: These results suggest that BGPs may be beneficial in the development and manufacture as potential therapeutic agents and functional foods.
- Subjects
ANTIOXIDANTS; POLYSACCHARIDES; GINSENG; THERAPEUTIC use of plant extracts; MOLECULAR weights
- Publication
Tropical Journal of Pharmaceutical Research, 2018, Vol 17, Issue 7, p1317
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v17i7.13