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- Title
Age- and Dose-Specific Anticonvulsant Action of Bumetanide in Immature Rats.
- Authors
Mareš, P.
- Abstract
GABA exhibits depolarizing action in the immature neurons due to high intracellular activity of chloride ions. It is maintained by cation-chloride cotransporter NKCC1 which is present in immature brain. Bumetanide is a specific inhibitor of this cotransporter. We studied possible anticonvulsant activity of bumetanide in pentylenetetrazol-induced seizures in three age groups of rat pups (7, 12, and 18 days old). Pretreatment with bumetanide (0.2-1 mg/kg i.p.) resulted in dose-dependent decrease of incidence of the tonic phase of generalized tonicclonic seizures in 12-day-old rats only. No effect was observed in younger and older animals. Higher dose of bumetanide (2.5 mg/kg) did not affect tonic convulsions but, on the contrary, decreased latencies of generalized seizures in 12-day-old animals. Lack of marked anticonvulsant effect is probably due to relative maturity of neurons in the brainstem where the generator of generalized seizures is localized. Age- and dosespecific suppression of the tonic phase needs further analysis.
- Subjects
ANTICONVULSANTS; SEIZURES (Medicine); BUMETANIDE; LABORATORY rats; ANIMAL models in research; BRAIN function localization; MOTOR neurons; GABA; PREVENTION; PHYSIOLOGY
- Publication
Physiological Research, 2009, Vol 58, Issue 6, p927
- ISSN
0862-8408
- Publication type
Article