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- Title
Tim-3 expression in glioma cells is associated with drug resistance.
- Authors
Zhang, Ji; Zhu, Zheng; Li, Yan; Zhuang, Qiu; Lai, Yuanhong; Li, Shao; Xu, Xiao; Liu, Jian; Zhu, Zheng Quan; Li, Yan Xia; Zhuang, Qiu Feng; Li, Shao Fang; Xu, Xiao Bing; Liu, Jian Min
- Abstract
<bold>Objective: </bold>T-cell immunoglobulin and mucin-domain containing-3 (Tim-3) has been widely recognized as a negative regulator of antitumor immunity. However, the mechanism by which Tim-3 suppresses antitumor treatment in gliomas remains unclear. This study aims to explore whether Tim-3 is expressed and to evaluate its effect in drug-fasted glioma cells.<bold>Subjects and Methods: </bold>U87 and U251 glioma cell lines were tested. Cell proliferation activity, cell viability, and the protein and mRNA levels of Tim-3 were detected using CCK-8, flow cytometry, Western blotting, and reverse transcription-quantitative polymerase chain reaction, respectively. Enhancement of the sensitivity of glioma cells to chemotherapeutic agents was tested after inhibiting Tim-3 expression using Tim-3 small interfering RNAs (siRNA).<bold>Results: </bold>As temozolomide (TMZ) concentration increased, the ratio of apoptotic cells also increased accordingly. However, the level of Tim-3 expression in living cells from the high-dose group was higher than in the low- and middle-dose groups. After interfering with the expression of Tim-3 using siRNA against Tim-3, the killing effect of TMZ rose through an increase in apoptosis.<bold>Conclusions: </bold>The presence of Tim-3 mRNA and protein in glioma cells was detected. Significantly, knocking down Tim-3 expression improved the potential of TMZ treatment.
- Subjects
SMALL interfering RNA; DRUG resistance; POLYMERASE chain reaction; CELLS; WESTERN immunoblotting
- Publication
Journal of Cancer Research & Therapeutics, 2019, Vol 15, Issue 4, p882
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/jcrt.JCRT_630_18