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- Title
A Neurotoxic Secretory Phospholipase A<sub>2</sub> Induces Apoptosis in Motoneuron-like Cells.
- Authors
Pražnikar, Zala Jenko; Petan, Toni; Pungerčar, Jože
- Abstract
Ammodytoxin A (AtxA) is a presynaptically neurotoxic secretory phospholipase A2 from snake venom. The aim of this study was to investigate the mechanism of its cytotoxicity expressed against mouse motoneuronal NSC34 cells. AtxA displayed a potent dose- and time-dependent cytotoxicity that was associated with apoptosis and not necrosis, as revealed by a reduction of mitochondrial membrane potential, activation of caspase-3, and by the absence of propidium iodide staining. The cytotoxic- and apoptosis-inducing effects of AtxA were specific for the motoneuronal cells; human embryonic kidney (HEK293) and mouse myoblast (C2C12) cells were shown to be resistant to the toxin.
- Subjects
NEUROTOXIC agents; PHOSPHOLIPASES; APOPTOSIS; MOTOR neurons; CELLS; EXOCYTOSIS; CELL physiology; CYTOLOGY; BIOLOGY
- Publication
Annals of the New York Academy of Sciences, 2009, Vol 1152, p215
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1111/j.1749-6632.2008.03999.x