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- Title
Reactive Oxygen Species Formation and Cell Death in Catalase-Deficient Tobacco Leaf Discs Exposed to Paraquat.
- Authors
Iannone, María; Rosales, Eliana; Groppa, María; Benavides, María
- Abstract
In the present work, the response of tobacco ( Nicotiana tabaccum L.) wild-type SR1 and transgenic CAT1AS plants (with a basal reduced CAT activity) was evaluated after exposure to the herbicide paraquat (PQ). Superoxide anion (O) formation was inhibited at 3 or 21 h of exposure, but HO production and ion leakage increased significantly, both in SR1 or CAT1AS leaf discs. NADPH oxidase activity was constitutively 57% lower in non-treated transgenic leaves than in SR1 leaves and was greatly reduced both at 3 or 21 h of PQ treatment. Superoxide dismutase (SOD) activity was significantly reduced by PQ after 21 h, showing a decrease from 70% to 55%, whereas catalase (CAT) activity decreased an average of 50% after 3 h of treatment, and of 90% after 21 h, in SR1 and CAT1AS, respectively. Concomitantly, total CAT protein content was shown to be reduced in non-treated CAT1AS plants compared to control SR1 leaf discs at both exposure times. PQ decreased CAT expression in SR1 or CAT1AS plants at 3 and 21 h of treatment. The mechanisms underlying PQ-induced cell death were possibly not related exclusively to ROS formation and oxidative stress in tobacco wild-type or transgenic plants.
- Subjects
TOBACCO; TRANSGENIC plants; PARAQUAT; HERBICIDES; CELL death; PLANTS
- Publication
Biological Trace Element Research, 2012, Vol 146, Issue 2, p246
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-011-9244-1