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- Title
The Mechanism of Liver Injury in Mice Caused by Lanthanoids.
- Authors
Min Fei; Na Li; Yuguan Ze; Jie Liu; Sisi Wang; Xiuaolan Gong; Yanmei Duan; Xiaoyang Zhao; Han Wang; Fashui Hong
- Abstract
It has been proven that higher dose of lanthanoid (Ln) can induce liver toxicities, but the mechanisms and the molecular pathogenesis are still unclear. In this study, LaCl, CeCl, and NdCl at a higher dose of 20 mg/kg body weight was injected into the abdominal cavity of ICR mice for 14 consecutive days, and the inflammatory responses of liver of mice were investigated by histopathological test, real-time quantitative reverse transcription polymerase chain reaction (RT-PCR), and enzyme-linked immunosorbent assay (ELISA) methods. The results showed the significant accumulation of Ln in the liver results in liver histopathological changes and, therefore, liver malfunctions. The real-time quantitative RT-PCR and ELISA analyses showed that Ln could significantly alter the mRNA and protein expressions of several inflammatory cytokines, including nucleic factor-κB, macrophage migration inhibitory factor, tumor necrosis factor-α, interleukin-1β, interleukin-6, cross-reaction protein, interleukin-4, and interleukin-10. Our results also implied that the inflammatory responses and liver injury likely are caused by 4f shell and alterable valence properties of Ln-induced liver toxicity.
- Subjects
LIVER injuries; RARE earth metals; ENZYME-linked immunosorbent assay; MICE; CYTOKINES; TUMOR necrosis factors
- Publication
Biological Trace Element Research, 2011, Vol 140, Issue 3, p317
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-010-8698-x