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- Title
Enteroendocrine profile of α-transducin immunoreactive cells in the gastrointestinal tract of the European sea bass (<i>Dicentrarchus labrax)</i>.
- Authors
Latorre, Rocco; Mazzoni, Maurizio; De Giorgio, Roberto; Vallorani, Claudia; Bonaldo, Alessio; Gatta, Pier Paolo; Corinaldesi, Roberto; Ruggeri, Eugenio; Bernardini, Chiara; Chiocchetti, Roberto; Sternini, Catia; Clavenzani, Paolo
- Abstract
In vertebrates, chemosensitivity of nutrients occurs through the activation of taste receptors coupled with G-protein subunits, including α-transducin (G αtran) and α-gustducin (G αgust). This study was aimed at characterising the cells expressing G αtran immunoreactivity throughout the mucosa of the sea bass gastrointestinal tract. G αtran immunoreactive cells were mainly found in the stomach, and a lower number of immunopositive cells were detected in the intestine. Some G αtran immunoreactive cells in the stomach contained G αgust immunoreactivity. Gastric G αtran immunoreactive cells co-expressed ghrelin, obestatin and 5-hydroxytryptamine immunoreactivity. In contrast, G αtran immunopositive cells did not contain somatostatin, gastrin/cholecystokinin, glucagon-like peptide-1, substance P or calcitonin gene-related peptide immunoreactivity in any investigated segments of the sea bass gastrointestinal tract. Specificity of G αtran and G αgust antisera was determined by Western blot analysis, which identified two bands at the theoretical molecular weight of ~45 and ~40 kDa, respectively, in sea bass gut tissue as well as in positive tissue, and by immunoblocking with the respective peptide, which prevented immunostaining. The results of the present study provide a molecular and morphological basis for a role of taste-related molecules in chemosensing in the sea bass gastrointestinal tract.
- Subjects
TRANSDUCIN; GASTROINTESTINAL system; SEA basses; VERTEBRATES; TASTE receptors; GHRELIN; SEROTONIN
- Publication
Fish Physiology & Biochemistry, 2013, Vol 39, Issue 6, p1555
- ISSN
0920-1742
- Publication type
Article
- DOI
10.1007/s10695-013-9808-4