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- Title
Detection of SARS-CoV-2 by RNAscope<sup>®</sup>in situ hybridization and immunohistochemistry techniques.
- Authors
Carossino, Mariano; Ip, Hon S.; Richt, Juergen A.; Shultz, Kendra; Harper, Kimberly; Loynachan, Alan T.; Del Piero, Fabio; Balasuriya, Udeni B. R.
- Abstract
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
- Subjects
IMMUNOHISTOCHEMISTRY techniques; SARS-CoV-2; IN situ hybridization; HISTOCHEMISTRY; VIRAL tropism; TROPISMS
- Publication
Archives of Virology, 2020, Vol 165, Issue 10, p2373
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-020-04737-w