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- Title
TNFα induces Caspase-3 activity in hematopoietic progenitor cells CD34+, CD33+, and CD41 + of myelodysplastic syndromes.
- Authors
Iriani, Anggraini; Rachman, Andhika; Setiabudy, Rahayuningsih D.; Kresno, Siti B.; Sudoyo, Aru W.; Arief, Mansyur; Harahap, Alida R.; Fatina, Marsya Kaila
- Abstract
Background: Cytopenia is the primary feature of Myelodysplastic Syndrome, even in the presence of hypercellular bone marrow. TNFα is recognized as both a proinflammatory, and proapoptotic cytokine with a well established role in promoting apoptosis in MDS. Therefore, TNFα has the potential to be a valuable biomarker for predicting the progression of cytopenia in MDS. This study aims to establish the role of TNFα exposure in triggering apoptosis through caspase-3 activity in CD34+, CD33+, and CD41 + cells in MDS. Methods: This study is an in vitro comparative experimental research. Bone marrow mononuclear cells were isolated as the source of hematopoietic progenitor cells. Subsequently, CD34+, CD33+, and CD41 + cells were exposed to rhTNFα, and the caspase-3 activity was measured using flowcytometry. Results: In MDS CD33 + and CD41 + caspase-3 activity of rhTNFα exposed cells was significantly higher than without exposed cells. The opposite result was found in CD34 + cells, where the caspase-3 activity without rhTNFα exposed cells was significantly higher than rhTNFα exposed cells. Conclusion: rhTNFα exposure led to an elevation in caspase-3 activity in MDS progenitor cells, especially in those that had differentiated into myeloid cell CD33 + and megakaryocyte cell CD41+, as opposed to the early progenitor cells CD34+.
- Subjects
PROGENITOR cells; MYELODYSPLASTIC syndromes; CD34 antigen; CASPASES; BONE marrow cells; APOPTOSIS
- Publication
BMC Molecular & Cell Biology, 2023, Vol 24, Issue 1, p1
- ISSN
2661-8850
- Publication type
Article
- DOI
10.1186/s12860-023-00495-0