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- Title
Clinical usefulness of multiplex PCR-lateral flow for the diagnosis of orthopedic-related infections.
- Authors
Yojiro Minegishi; Katsufumi Uchiyama; Keizo Sakurai; Shiro Ibe; Hiromi Kanda; Shin Nihonyanagi; Masaki Nakamura; Shinsuke Ikeda; Masashi Takaso
- Abstract
Objectives: Polymerase chain reaction (PCR)-based assays are being increasingly used for the diagnosis of orthopedic-related infections. Unfortunately, classical PCR requires imaging devices that are expensive and complex. We previously developed the PCR-lateral flow (PCR-LF) method, which does not require any additional imaging device. In the present study, the objective was to determine whether PCR-LF tests could be used to effectively diagnose orthopedic-related infections. Methods: In this study, we used PCR-LF to diagnose common causes of orthopedic-related infections and compared the results to those from conventional bacterial cultures of the same samples. Results: Notably, for 228 synovial fluid or pus specimens, the sensitivity and specificity of bacterial cultures were 53.5% and 97.7%, respectively, compared to 61.6% and 89.9% for PCR-LF. Although the difference in sensitivity between bacterial cultures and PCR-LF was not significant, when our analysis was limited to cases with suspected periprosthetic joint infection, the sensitivity of PCR-LF (66.1%) was superior to that of bacterial cultures (42.9%). Conclusion: This study indicates that PCR-LF is a useful method for diagnosing orthopedicrelated infections.
- Subjects
POLYMERASE chain reaction; BACTERIAL cultures; INFECTION; HEALTH outcome assessment; DIAGNOSIS
- Publication
Modern Rheumatology, 2019, Vol 29, Issue 5, p867
- ISSN
1439-7595
- Publication type
Article
- DOI
10.1080/14397595.2018.1514690