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- Title
Effects of isolated bacteria application on chemical composition and fermentation characteristic of rye silage.
- Authors
Joo, Y. H.; Lee, H. J.; Lee, S. S.; Han, O. K.; Kim, S. C.
- Abstract
This study was conducted to estimate the effect of 2 isolated bacteria on chemical composition and fermentation characteristics of rye silage harvested at 20 d after heading stage. Two microbes, which had confirmed the effect of antifungal activity (Lactobacillus brevis [LB]) or fibrinolysis ability (Leuconostoc holzapfelii [LH]), were selected from 832 dominant microbes by the tests of antimicrobial activities against fungi and fibrinolysis ability with 3 enzyme plate assay (cellulase, xylanase, and esterase). Rye forage was harvested at 20 d (27.3% DM) after heading stage, chopped into 3 to 5 cm lengths, and divided into 4 treatments: applications of 2 mL/kg of distilled water (CON), 1.2 × 105 cfu/g of LB, 1.0 × 105 cfu/g of LH, or combo of LB and LH at 1:1 ratio (MIX). The forages with applied bacterial additives were ensiled into 10-L mini-silo with 4 replications for 100 d. The bacterial additives were not affected on chemical composition and in vitro digestibility of rye forages. However, all silages with applied bacterial additives had lower NDF concentrations (P < 0.05; 67.1 vs. 69.0%) than CON silage whereas only AT silage had lower ADF concentrations (P < 0.05; 41.6 vs. 43.4%). The silages with LH alone and combo with LB applied had higher (P < 0.05; 67.5 vs. 63.2%) in vitro DM digestibility than the other silages, whereas bacterial additives were not affected on in vitro NDF digestibility. The pH was lowest (P < 0.05, 4.26 vs. 5.04) in LB and MIX silages but highest in CON silage. In contrast, lactate concentration was higher (P < 0.05; 5.36 vs. 0.50%) in all silages with bacterial additives applied than in CON silage. Silages with LB and MIX applied had higher acetate concentrations (P < 0.05; 5.43 vs. 2.82%) than the other silages, whereas silages with LH and MIX applied had lower propionate concentrations (P < 0.05; 0.35 vs. 0.66%) than CON silage. Lactic acid bacteria (6.75 vs. 5.13 log10 cfu/g) and yeast (6.56 vs. 5.42 log10 cfu/g) were more abundant (P < 0.05) in CON and LH silages than in LB and MIX silages, whereas mold was not detected in any silages. Therefore, it could be concluded that LB is more effective on antifungal activity by the results of lower pH and yeast but higher lactate and acetate concentrations. And again, LH is more effective on fibrinolysis ability by the results of lower NDF concentration but higher in vitro DM digestibility.
- Subjects
RYE as feed; LACTOBACILLUS brevis; ANTIFUNGAL agents
- Publication
Journal of Animal Science, 2017, Vol 95, p141
- ISSN
0021-8812
- Publication type
Abstract
- DOI
10.2527/asasann.2017.285