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- Title
The SUMO protease SENP6 is essential for inner kinetochore assembly.
- Authors
Mukhopadhyay, Debaditya; Arnaoutov, Alexei; Dasso, Mary
- Abstract
We have analyzed the mitotic function of SENP6, a small ubiquitin-like modifier (SUMO) protease that disassembles conjugated SUMO-2/3 chains. Cells lacking SENP6 showed defects in spindle assembly and metaphase chromosome congression. Analysis of kinetochore composition in these cells revealed that a subset of proteins became undetectable on inner kinetochores after SENP6 depletion, particularly the CENP-H/I/K complex, whereas other changes in kinetochore composition mimicked defects previously reported to result from CENP-H/I/K depletion. We further found that CENP-I is degraded through the action of RNF4, a ubiquitin ligase which targets polysumoylated proteins for proteasomal degradation, and that SENP6 stabilizes CENP-I by antagonizing RNF4. Together, these findings reveal a novel mechanism whereby the finely balanced activities of SENP6 and RNF4 control vertebrate kinetochore assembly through SUMO-targeted destabilization of inner plate components.
- Subjects
PROTEOLYTIC enzymes; UBIQUITIN; MITOSIS; SPINDLE apparatus; PROTEINS; LIGASES
- Publication
Journal of Cell Biology, 2010, Vol 188, Issue 5, p681
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200909008