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- Title
Differential contribution of Budσp and Kar9p to microtubule capture and spindle orientation in S. cerevisiae.
- Authors
Huisman, Stepnen M.; Bales, Olivia A.M.; Bertrana, Marie; Smeets, Monique F. M. A.; Reed, Steven I.; Segal, Marisa
- Abstract
In Sacchczromyces cerevisice, spindle orientation is controlled by a temporal and spatial program of microtubule (MT)-cortex interactions. This program requires Bud6p/Aip3p to direct the old pole to the bud and confine the new pole to the mother cell. Bud6p function has been linked to Kar9p, a protein guiding MTs along actin cables. Here, we show that Kar9p does not mediate Bud6p functions in spindle orientation. Based on live microscopy analysis, kar9δ cells maintained Bud6p-dependent MT capture. Conversely, bud6δ cells supported Kar9p-associated MT delivery to the bud. Moreover, additive phenotypes in budδ kar9δ or bud6δ dyn1δ mutants underscored the separate contributions of Bud6p, Kar9p, and dynein to spindle positioning. Finally, tub2C545, a mutation decreasing MT dynamics, sup- pressed a kar9δ mutation in a BUD6-dependent manner. Thus, Kar9p-independent capture at Bud6p sites can effect spindle orientation provided MT turnover is reduced. Together, these results demonstrate Bud6p function in MT capture at the cell cortex, independent of Kar9p-mediated MT delivery along actin cables.
- Subjects
MICROTUBULES; ORGANELLES; ACTIN; PHENOTYPES; CELLS; CELL nuclei
- Publication
Journal of Cell Biology, 2004, Vol 167, Issue 2, p231
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200407167