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- Title
A new organotypic model containing dermal-type macrophages.
- Authors
Bechetoille, Nicolas; Vachon, Hortense; Gaydon, Amandine; Boher, Aurelie; Fontaine, Thomas; Schaeffer, Evelyne; Decossas, Marion; André-Frei, Valérie; Mueller, Christopher G.
- Abstract
Human skin equivalents (SEs) are popular three-dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti-inflammatory dermal macrophages has been performed. Here, we show that monocyte-derived dermal-type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. They maintain their cell surface markers CD163, DC-SIGN/CD209 and HLA-DR, which discriminate them from monocytes and dendritic cells. They retain the ability to produce the anti-inflammatory cytokine IL-10 in response to lipopolysaccharide (LPS) and to phagocytose latex beads. We thus demonstrate the feasibility of creating macrophage-fibroblast 3D cultures as a first step towards generating SEs with dermal macrophages.
- Subjects
MACROPHAGES; CELL culture; FIBROBLASTS; DENDRITIC cells; MONOCYTES; CYTOKINES; ENDOTOXINS
- Publication
Experimental Dermatology, 2011, Vol 20, Issue 12, p1035
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/j.1600-0625.2011.01383.x