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- Title
Effect of exogenous apo E on the cellular binding of lipoproteins.
- Authors
Mokuno, H; Ishibashi, S; Shimano, H; Gothoda, T; Takaku, F; Yamada, N
- Abstract
It is well known that apolipoprotein E (apo E) has a significant role in the metabolism of plasma cholesterol through receptor-mediated endocytosis of lipoproteins containing apo B 100. We have reported that intravenous administration of apo E increases the clearance of lipoproteins containing apo B 100, resulting in reduced plasma cholesterol levels in WHHL rabbits. In the present study, we investigated the effects of exogenous apo E on lipoprotein binding to cell surface receptors. Lipoproteins were enriched in apo E by the incubation with purified apo E. Among lipoproteins enriched in apo E, VLDL and intermediate-density lipoprotein demonstrated higher binding affinity to cell surface receptor on fibroblast than respective unmodified lipoproteins. In competitive binding study, VLDL enriched by apo E (VLDL + E) completely suppressed 125I-LDL binding to human skin fibroblasts and showed the greatest binding activity among different lipoprotein classes, whereas both VLDL and LDL incompletely suppressed a binding of 125I-VLDL + E. This result indicated that apo-E-rich VLDL can bind not only to LDL receptor but to binding sites other than LDL receptors.
- Subjects
ANIMAL experimentation; APOLIPOPROTEINS; BINDING sites; CELL receptors; LOW density lipoproteins; RABBITS
- Publication
Gerontology, 1990, Vol 36, Issue 1, p42
- ISSN
0304-324X
- Publication type
journal article