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- Title
Strategies for improvement of cloning by somatic cell nuclear transfer.
- Authors
Qiu, Xiaoyan; Xiao, Xiong; Martin, Graeme B.; Li, Nan; Ling, Wenhui; Wang, Mingyu; Li, Yuemin
- Abstract
Somatic cell nuclear transfer (SCNT) is a powerful tool that is being applied in a variety of fields as diverse as the cloning and production of transgenic animals, rescue of endangered species and regenerative medicine. However, cloning efficiency is still very low and SCNT embryos generally show poor developmental competency and many abnormalities. The low efficiency is probably due to incomplete reprogramming of the donor nucleus and most of the developmental problems are thought to be caused by epigenetic defects. Applications of SCNT will, therefore, depend on improvements in the efficiency of production of healthy clones. This review has summarised the progress and strategies that have been used to make improvements in various animal species, especially over the period 2010–2017, including strategies based on histone modification, embryo aggregation and mitochondrial function. There has been considerable investiagation into the mechanisms that underpin each strategy, helping us better understand the nature of genomic reprogramming and nucleus–cytoplasm interactions. The successful application of somatic cell nuclear transfer (SCNT) to a broad variety of species requires an improvement in its efficiency. We have seen, especially during the past 5 years, several technical strategies for improving SCNT, including strategies based on histone modification, embryo aggregation and mitochondrial function. These strategies will ultimately allow the promotion of SCNT technology to practical purposes.
- Subjects
SOMATIC cell nuclear transfer; TRANSGENIC animals
- Publication
Animal Production Science, 2019, Vol 59, Issue 7, p1218
- ISSN
1836-0939
- Publication type
Article
- DOI
10.1071/AN17621