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- Title
GC Content of Early Metazoan Genes and Its Impact on Gene Expression Levels in Mammalian Cell Lines.
- Authors
Gul, Ismail Sahin; Staal, Jens; Hulpiau, Paco; De Keuckelaere, Evi; Kai Kamm; Deroo, Tom; Sanders, Ellen; Staes, Katrien; Driege, Yasmine; Saeys, Yvan; Beyaert, Rudi; Technau, Ulrich; Schierwater, Bernd; van Roy, Frans
- Abstract
With the genomes available for many animal clades, including the early-branching metazoans, one can readily study the functional conservation of genes across a diversity of animal lineages. Ectopic expression of an animal protein in, for instance, amammalian cell line is a generally used strategy in structure-function analysis. However, thismight turn out to be problematic in case of distantly related species. Here we analyzed the GC content of the coding sequences of basal animals and show its impact on gene expression levels in human cell lines, and, importantly, how this expression efficiency can be improved. Optimization of the GC3 content in the coding sequences of cadherin, alpha-catenin, and paracaspase of Trichoplax adhaerens dramatically increased the expression of these basal animal genes in human cell lines.
- Subjects
METAZOA; GENE expression in mammals; CELL lines; CADHERINS; BIOLOGICAL evolution
- Publication
Genome Biology & Evolution, 2018, Vol 10, Issue 3, p909
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evy040