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- Title
Analysis of the Optimal Blood Sampling Conditions for Estimation of Hematopoietic Progenitor Cell Count by the SE-9000 Automated Hematolyzer.
- Authors
Nomura, Tsutomu; Kubota, Yoshitsugu; Baba, Natsumi; Saigo, Katsuyasu; Ohnishi, Hiroaki; Kitanaka, Akira; Taminato, Tomohiko
- Abstract
We evaluated the optimal conditions for blood sampling for hematopoietic progenitor cells (HPCs) as estimated by the immature information program of the SE-9000 automated hematology analyzer. The HPC count was most stable when the blood samples were incubated at room temperature with ethylene-diaminetetraacetic acid dipotassium (EDTA-2K) as an anticoagulant. The HPC count should, however, be measured within 4 h after blood collection, even under optimal conditions. In contrast, the CD34+ cell count estimated by flow cytometric analysis was stable for at least 21 h after the blood samples were incubated with EDTA-2K at room temperature or 4°C. When appropriate blood samples were used, the HPC count in the peripheral blood significantly correlated with the CD34+ cell count in the peripheral blood and in the apheresis yields (r = 0.798 and 0.635, respectively); therefore, the HPC count is a reliable predictor for initiation of apheresis procedures to obtain sufficient HPCs for peripheral blood stem cell transplantation. Copyright © 2004 S. Karger AG, Basel
- Subjects
BLOOD testing; BIOLOGICAL apparatus &; supplies; SCIENTIFIC apparatus &; instruments; HEMATOLOGY; HEMATOPOIETIC system
- Publication
Acta Haematologica, 2004, Vol 112, Issue 3, p152
- ISSN
0001-5792
- Publication type
Article
- DOI
10.1159/000079727