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- Title
Novel Observations From Next-Generation RNA Sequencing of Highly Purified Human Adult and Fetal Islet Cell Subsets.
- Authors
Blodgett, David M.; Nowosielska, Anetta; Afik, Shaked; Pechhold, Susanne; Cura, Anthony J.; Kennedy, Norman J.; Soyoung Kim; Kucukural, Alper; Davis, Roger J.; Kent, Sally C.; Greiner, Dale L.; Garber, Manuel G.; Harlan, David M.; diIorio, Philip
- Abstract
Understanding distinct gene expression patterns of normal adult and developing fetal human pancreatic α- and β-cells is crucial for developing stem cell therapies, islet regeneration strategies, and therapies designed to increase β-cell function in patients with diabetes (type 1 or 2). Toward that end, we have developed methods to highly purify α-, β-, and δ-cells from human fetal and adult pancreata by intracellular staining for the cell-specific hormone content, sorting the subpopulations by flow cytometry, and, using next-generation RNA sequencing, we report the detailed transcriptomes of fetal and adult α- and β-cells. We observed that human islet composition was not influenced by age, sex, or BMI, and transcripts for inflammatory gene products were noted in fetal β-cells. In addition, within highly purified adult glucagon-expressing α-cells, we observed surprisingly high insulin mRNA expression, but not insulin protein expression. This transcriptome analysis from highly purified islet α- and β-cell subsets from fetal and adult pancreata offers clear implications for strategies that seek to increase insulin expression in type 1 and type 2 diabetes.
- Subjects
PANCREATIC beta cells; RNA sequencing; ISLANDS of Langerhans; GENE expression; GENETICS of diabetes
- Publication
Diabetes, 2015, Vol 64, Issue 9, p3172
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/db15-0039