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- Title
Long Interspersed Nuclear Element-1 Analytes in Extracellular Vesicles as Tools for Molecular Diagnostics of Non-Small Cell Lung Cancer.
- Authors
Bowers, Emma C.; Cavalcante, Alexandre M.; Nguyen, Kimberly; Li, Can; Wang, Yingshan; El-Zein, Randa; Chen, Shu-Hsia; Kim, Min P.; McKay, Brian S.; Ramos, Kenneth S.
- Abstract
Aberrant expression of the oncogenic retrotransposon LINE-1 is a hallmark of various cancer types, including non-small cell lung cancers (NSCLCs). Here, we present proof-of-principle evidence that LINE-1 analytes in extracellular vesicles (EVs) serve as tools for molecular diagnostics of NSCLC, with LINE-1 status in tumor cells and tissues mirroring the LINE-1 mRNA and ORF1p cargos of EVs from lung cancer cell culture conditioned media or human plasma. The levels of LINE-1 analytes in plasma EVs from ostensibly healthy individuals were higher in females than males. While the profiles of LINE-1 mRNA and ORF1p in African Americans compared to Hispanics were not significantly different, African Americans showed slightly higher ORF1p content, and 2–3 times greater ranges of LINE-1 values compared to Hispanics. Whole plasma ORF1p levels correlated with EV ORF1p levels, indicating that most of the circulating LINE-1 protein is contained within EVs. EV LINE-1 mRNA levels were elevated in patients with advanced cancer stages and in select patients with squamous cell carcinoma and metastatic tumors compared to adenocarcinomas. The observed EV LINE-1 mRNA profiles paralleled the patterns of ORF1p expression in NSCLC tissue sections suggesting that LINE-1 analytes in plasma EVs may serve to monitor the activity of LINE-1 retroelements in lung cancer.
- Subjects
NON-small-cell lung carcinoma; EXTRACELLULAR vesicles; MOLECULAR diagnosis; CANCER patients; CANCER cell culture; VESICLES (Cytology); CELL culture
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 2, p1169
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25021169