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- Title
Genistein Promotes Skeletal Muscle Regeneration by Regulating miR-221/222.
- Authors
Shen, Linyuan; Liao, Tianci; Chen, Jingyun; Ma, Jianfeng; Wang, Jinyong; Chen, Lei; Zhang, Shunhua; Zhao, Ye; Niu, Lili; Zeng, Changjun; Gan, Mailin; Zhu, Li
- Abstract
Genistein (GEN), a phytoestrogen, has been reported to regulate skeletal muscle endocrine factor expression and muscle fiber type switching, but its role in skeletal muscle regeneration is poorly understood. As a class of epigenetic regulators widely involved in skeletal muscle development, microRNAs (miRNAs) have the potential to treat skeletal muscle injury. In this study, we identified miR-221 and miR-222 and their target genes MyoG and Tnnc1 as key regulators during skeletal muscle regeneration, and both were regulated by GEN. C2C12 myoblasts and C2C12 myotubes were then used to simulate the proliferation and differentiation of muscle satellite cells during skeletal muscle regeneration. The results showed that GEN could inhibit the proliferation of satellite cells and promote the differentiation of satellite cells by inhibiting the expression of miR-221/222. Subsequent in vitro and in vivo experiments showed that GEN improved skeletal muscle regeneration mainly by promoting satellite cell differentiation in the middle and late stages, by regulating miR-221/222 expression. These results suggest that miR-221/222 and their natural regulator GEN have potential applications in skeletal muscle regeneration.
- Subjects
MYOBLASTS; MUSCLE regeneration; SKELETAL muscle; GENISTEIN; SKELETAL muscle injuries; SATELLITE cells; CELL differentiation
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 21, p13482
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms232113482