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- Title
Elucidating the Functional Roles of Helper and Cytotoxic T Cells in the Cell-Mediated Immune Responses of Olive Flounder (Paralichthys olivaceus).
- Authors
Jung, Jae Wook; Lee, Ae Rin; Kim, Jaesung; Kim, Young Rim; Lazarte, Jassy Mary S.; Lee, Jung Suk; Thompson, Kim D.; Kim, Hyeongsu; Jung, Tae Sung
- Abstract
In higher vertebrates, helper and cytotoxic T cells, referred to as CD4 and CD8 T lymphocytes, respectively, are mainly associated with adaptive immunity. The adaptive immune system in teleosts involves T cells equivalent to those found in mammals. We previously generated monoclonal antibodies (mAbs) against olive flounder (Paralichthys olivaceus) CD4 T cells, CD4-1 and CD4-2, and used these to describe the olive flounder's CD4 Tcell response during a viral infection. In the present study, we successfully produced mAbs against CD8 T lymphocytes and their specificities were confirmed using immuno-blotting, immunofluorescence staining, flow cytometry analysis andreverse transcription polymerase chain reaction (RT-PCR). The results showed that these mAbs are specific for CD8 T lymphocytes. We also investigated variations in CD4 and CD8 T cells populations, and analyzed the expression of immune-related genes expressed by these cells in fish infected with nervous necrosis virus or immunized with thymus dependent and independent antigens. We found that both CD4 and CD8 T lymphocyte populations significantly increased in these fish and Th1-related genes were up-regulated compared to the control group. Collectively, these findings suggest that the CD4 and CD8 T lymphocytes in olive flounder are similar to the helper and cytotoxic T cells found in mammals, and Th1 and cytotoxic immune responses are primarily involved in the early adaptive immune response against extracellular antigens.
- Subjects
CYTOTOXIC T cells; T cells; PARALICHTHYS; IMMUNE response; CELL populations; T helper cells; MONOCLONAL antibodies
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 2, p847
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22020847