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- Title
Signaling Through Purinergic Receptor P2Y2 Enhances Macrophage IL-1β Production.
- Authors
de la Rosa, Gonzalo; Gómez, Ana I.; Baños, María C.; Pelegrín, Pablo
- Abstract
The release of nucleotides during necrosis or apoptosis has been described to have both proinflammatory and anti-inflammatory effect on the surrounding cells. Here we describe how low concentrations of UTP and ATP applied during macrophage priming enhance IL-1β production when subsequently the NLRP3 inflammasome is activated in murine resident peritoneal macrophages. Deficiency or pharmacological inhibition of the purinergic receptor P2Y2 reverted the increase of IL-1β release induced by nucleotides. IL-1β increase was found dependent on the expression of Il1b gene and probably involving JNK activity. On the contrary, nucleotides decreased the production of a different proinflammatory cytokines such as TNF-α. These results suggest that nucleotides could shape the response of macrophages to obtain a unique proinflammatory signature that might be relevant in unrevealing specific inflammatory conditions.
- Subjects
PURINERGIC receptors; PERITONEAL macrophages; APOPTOSIS; NUCLEOTIDES
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 13, p4686
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21134686