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- Title
Protective effect of calcitonin on lumbar fusion-induced adjacent-segment disc degeneration in ovariectomized rat.
- Authors
Chang-Cheng Liu; Fa-Ming Tian; Zhuang Zhou; Peng Wang; Yu Gou; Heng Zhang; Wen-Ya Wang; Yong Shen; Ying-Ze Zhang; Liu Zhang; Liu, Chang-Cheng; Tian, Fa-Ming; Zhou, Zhuang; Wang, Peng; Gou, Yu; Zhang, Heng; Wang, Wen-Ya; Shen, Yong; Zhang, Ying-Ze; Zhang, Liu
- Abstract
<bold>Background: </bold>Intervertebral disc (IVD) degeneration and pathological changes in the spinal cord are major causes of back pain. In addition to its well-established anti-resorptive effect on bone, calcitonin (CT) potentially exerts protective effects on IVD degeneration in ovariectomized rats. However, possible therapeutic effects of CT on lumbar fusion-induced adjacent-segment disc degeneration (ASDD) have not been investigated yet. In this study, we examined the effects of CT on IVD degeneration adjacent to a lumbar fusion in ovariectomized rats.<bold>Methods: </bold>Posterolateral lumbar fusion (PLF) at L4-5 was performed 4 weeks after ovariectomy (OVX) or sham surgery in female Sprague-Dawley rats. Following PLF + OVX, rats received either salmon CT (OVX + PLF + sCT, 16 IU/Kg/2d) or vehicle (OVX + PLF + V) treatment for 12 weeks; the remaining rats were divided into Sham + V, OVX + V, and PLF + V groups. Fusion status was analyzed by manual palpation and radiography. Adjacent segment disc was assessed by histological, histomorphometric, immunohistochemical analysis. L6 vertebrae microstructures were evaluated by micro-computed tomography.<bold>Results: </bold>Histological analysis showed more severe ASDD occurred in OVX + PLF + V rats compared with the OVX + V or PLF + V groups. CT treatment suppressed the score for ASDD, increased disc height, and decreased the area of endplate calcification. Immunohistochemical staining demonstrated that CT decreased the expression of collagen type-I, matrix metalloproteinase-13, and a disintegrin and metalloproteinase with thrombospondin motifs-4, whereas it increased the expression of collagen type-II and aggrecan in the disc. Micro-computed tomography indicated that CT increased bone mass and improved the microstructure of the L6 vertebrae.<bold>Conclusions: </bold>These results suggest that CT can prevent ASDD, induce beneficial changes in IVD metabolism, and inhibit deterioration of the trabecular microarchitecture of vertebrae in osteoporotic rats with lumbar fusion.
- Subjects
CALCITONIN; LUMBAR vertebrae; INTERVERTEBRAL disk diseases; INTERVERTEBRAL disk displacement; OVARIECTOMY; IMMUNOSTAINING; LABORATORY rats; PREVENTION; ANIMAL experimentation; COMPUTED tomography; DIPHOSPHONATES; IMMUNOHISTOCHEMISTRY; RATS; SPINE diseases; SPINAL fusion; BONE density; PHARMACODYNAMICS; THERAPEUTICS
- Publication
BMC Musculoskeletal Disorders, 2015, Vol 16, p1
- ISSN
1471-2474
- Publication type
journal article
- DOI
10.1186/s12891-015-0788-7