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- Title
Metabolic pathway analysis of glycerol metabolism in Klebsiella pneumoniae incorporating oxygen regulatory system.
- Authors
Zhang, Qingrui; Xiu, Zhilong
- Abstract
It is well known that environmental and genetic perturbations have major effects on the metabolic behavior of cells. In this work, a model that utilizes existing knowledge of oxygen and redox sensing/regulatory system to assist elementary flux modes (EFMs) has been developed and was carried out to predict the metabolic potential of Klebsiella pneumoniae for the production of 1,3-propanediol (1,3-PD) under anaerobic and aerobic conditions. It was found that the theoretical optimal 1,3-PD yield could reach to 0.844 mol mol−1 if the pentose phosphate pathway (PPP), and transhydrogenase had a high flux under anaerobic condition. However, PPP had little influence on the theoretical 1,3-PD yield, and the flux through tricarboxylic acid (TCA) cycle was high under aerobic conditions. In addition, the effect of oxygen level on the 1,3-PD and biomass was further analyzed. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
- Subjects
KLEBSIELLA pneumoniae; CELLS; GLYCERIN; METABOLIC regulation; OXYGEN; TRICARBOXYLIC acids
- Publication
Biotechnology Progress, 2009, Vol 25, Issue 1, p103
- ISSN
8756-7938
- Publication type
Article
- DOI
10.1002/btpr.70