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- Title
Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells.
- Authors
Jienny Lee; Keum Sil Lee; Chan-Lan Kim; Jeong Su Byeon; Na-Yeon Gu; In-Soo Cho; Sang-Ho Cha
- Abstract
Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs and the cells were subjected to sequential sub-passaging from passage 1 (P1) to P7. Canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics, expression of molecules associated with self-renewal, expression of cell surface markers and differentiation potentials at P3. Cumulative population doubling level was significantly higher in cAD-MSCs of young donors than in those of old donors. In addition, expressions of CD73, CD80, Oct3/4, Nanog, cell survival genes and differentiation potentials were significantly higher in young donors than in old donors. The present study suggests that donor age should be considered when developing cell-based therapies for clinical application of cAD-MSCs
- Subjects
MESENCHYMAL stem cells; ADIPOSE tissues; CANIDAE; GENE expression; CELL differentiation
- Publication
Journal of Veterinary Science, 2017, Vol 18, Issue 2, p141
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2017.18.2.141