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- Title
A novel membrane based process to isolate photosystem-I membrane complex from spinach.
- Authors
Jianguo Liu; Mengmeng Yin; Meng Wang; Xuefang Zhang; Baosheng Ge; Shuang Liu; Jianren Lu; Zhanfeng Cui
- Abstract
The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300 kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 and n-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I were also studied using an O electrode. Under optimized conditions, the PS-I purity obtained in the retentate was about 84% and the activity recovery was greater than 94% after ultrafiltration. To our knowledge, this is the first report of the isolation of a membrane protein using ultrafiltration alone.
- Subjects
SPINACH; ULTRAFILTRATION; MEMBRANE proteins; BIOLOGICAL membranes; PROTEINS
- Publication
Photosynthesis Research, 2011, Vol 107, Issue 2, p187
- ISSN
0166-8595
- Publication type
Article
- DOI
10.1007/s11120-011-9625-5