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- Title
Self-assembled gold nanocrystal micelles act as an excellent artificial nanozyme with ribonuclease activity.
- Authors
Zhiming Zhang; Qiuan Fu; Xiangqiu Li; Xin Huang; Jiayun Xu; Jiacong Shen; Junqiu Liu
- Abstract
Water-soluble Au nanocrystal (NC) micelles with an inserted catalytic Cu(II) center that act as excellent nanoenzyme models for imitating ribonuclease were constructed by supramolecular self-assembly. The dodecane-1-thiol-based Au NC was constructed first, and subsequently the cationic surfactant hexadecyltrimethylammonium bromide and the catalytic ligand ( N1, N1-bis(2-aminoethyl)- N2-dodecylethane-1,2-diamine) copper(II) were installed on the surface of the Au NC via hydrophobic interaction. The catalytic capability of the Au NC micelles designed was estimated by the cleavage of a typical RNA analogue, 2-hydroxypropyl p-nitrophenyl phosphate (HPNP). The study of the catalytic behavior of Au NC micelle catalysis showed that the Au NC micelles exhibited dramatic ribonuclease-like activity: a high rate acceleration of kcat/ kuncat = 1.10 × 105 for the cleavage of HPNP in comparison with the spontaneous cleavage of HPNP ( kuncat) was observed. The catalytic capability for HPNP cleavage by these functionalized Au NC micelles can be compared with that of covalent Au nanoparticles reported previously as nanozymes under comparable conditions. A detailed investigation of enzymatic kinetics was carried out and a possible mechanism was suggested. [Figure not available: see fulltext.]
- Subjects
RIBONUCLEASES; NANOCRYSTALS; MICELLES; COLLOIDS; BIOINORGANIC chemistry
- Publication
Journal of Biological Inorganic Chemistry (JBIC), 2009, Vol 14, Issue 5, p653
- ISSN
0949-8257
- Publication type
Article
- DOI
10.1007/s00775-009-0478-8