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- Title
Spatial transcriptomics reveal topological immune landscapes of Asian head and neck angiosarcoma.
- Authors
Loh, Jui Wan; Lee, Jing Yi; Lim, Abner Herbert; Guan, Peiyong; Lim, Boon Yee; Kannan, Bavani; Lee, Elizabeth Chun Yong; Gu, Ning Xin; Ko, Tun Kiat; Ng, Cedric Chuan-Young; Lim, Jeffrey Chun Tatt; Yeong, Joe; Lim, Jing Quan; Ong, Choon Kiat; Teh, Bin Tean; Chan, Jason Yongsheng
- Abstract
Angiosarcomas are rare malignant tumors of the endothelium, arising commonly from the head and neck region (AS-HN) and recently associated with ultraviolet (UV) exposure and human herpesvirus-7 infection. We examined 81 cases of angiosarcomas, including 47 cases of AS-HN, integrating information from whole genome sequencing, gene expression profiling and spatial transcriptomics (10X Visium). In the AS-HN cohort, we observed recurrent somatic mutations in CSMD3 (18%), LRP1B (18%), MUC16 (18%), POT1 (16%) and TP53 (16%). UV-positive AS-HN harbored significantly higher tumor mutation burden than UV-negative cases (p = 0.0294). NanoString profiling identified three clusters with distinct tumor inflammation signature scores (p < 0.001). Spatial transcriptomics revealed topological profiles of the tumor microenvironment, identifying dominant but tumor-excluded inflammatory signals in immune-hot cases and immune foci even in otherwise immune-cold cases. In conclusion, spatial transcriptomics reveal the tumor immune landscape of angiosarcoma, and in combination with multi-omic information, may improve implementation of treatment strategies. A spatial transcriptomic and genomic analysis of angiosarcomas, including samples from the head and neck region, reveal the tumor immune landscape of this cancer.
- Subjects
SOMATIC mutation; ANGIOSARCOMA; GENE expression profiling; WHOLE genome sequencing; GENOMICS; ENDOTHELIUM; LANDSCAPES
- Publication
Communications Biology, 2023, Vol 6, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-023-04856-5