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- Title
Potent in vitro Activity of the Albumin Fusion Type 1 Interferons (Albumin-Interferon-Alpha and Albumin-Interferon-Beta) against RNA Viral Agents of Bioterrorism and the Severe Acute Respiratory Syndrome (SARS) Virus.
- Authors
Subramanian, G. Mani; Moore, Paul A.; Gowen, Brian B.; Olsen, Aaron L.; Barnard, Dale L.; Paragas, Jason; Hogan, Robert J.; Sidwell, Robert W.
- Abstract
Background: The type 1 interferons (INF-α and INF-β) are potent antiviral agents. Albumin-INF-α and albumin-INF-β are novel recombinant proteins consisting of IFN-α or IFN-β genetically fused to human albumin. Methods: The in vitro antiviral activity of albumin-IFN-α was evaluated against representative bioterrorism viral agents and the severe acute respiratory syndrome virus. Antiviral activity was assessed using inhibition of cytopathic effect and neutral red staining. Results: EC50 values for albumin-IFN-α ranged from <0.1 ng/ml for Punta Toro virus to 65 ng/ml for Venezuelan equine encephalitis virus in the neutral red assay. Albumin-IFN-β showed 75- and 360-fold greater in vitro activity than albumin-IFN-α against Ebola virus and severe acute respiratory syndrome, respectively. Conclusion: Further evaluation of these long-acting albumin-IFN fusion proteins as prophylactic or therapeutic agents against these viral agents of bioterrorism in relevant primate models is warranted. Copyright © 2008 S. Karger AG, Basel
- Subjects
ALBUMINS; GENETICS; RESPIRATORY diseases; ANTIVIRAL agents; BIOTERRORISM
- Publication
Chemotherapy (0009-3157), 2008, Vol 54, Issue 3, p176
- ISSN
0009-3157
- Publication type
Article
- DOI
10.1159/000140361