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- Title
RESULTS REGARDING THE CLASSICAL AND MODERN PATHOGEN ELIMINATION TECHNIQUES OF PLUM POX VIRUS AT PLUM (PRUNUS DOMESTICA, L.).
- Authors
Jakab-Ilyefalvi, Zs.; Pamfil, D.
- Abstract
In international and national certification schemes of fruit-stock material production a particularly important role is occupied by the elimination of recalcitrant viral pathogens and also by the serological and molecular diagnostics in each propagation stage. In order to align with international standards, in Romania appeared the urgent need to create superior nuclear stock material, for both pome and stone species because currently there are shortcomings in this field of horticulture. The Romanian certification scheme under the Order 1295/2005 indicated the possibility of pathogen elimination by thermotherapy. Our research sought to make original scientific contributions in both the classical method of pathogen elimination and in the in vitro method of infected clone heads (MAPDR Order 1295, 2005). Studying as biological material the plum, a traditional stone fruit growing species in Romania, we have chosen as a central objective of the researches to create an optimized methodology to eliminate the most important recalcitrant viral pathogens (PPV) of this species with negative economic effects in production. According to our studies the most effective pathogen elimination method is the combined methodology of tissue cultures mediated by the application of antiviral agent Ribavirin, hence the in vitro chemotherapy procedure, in terms of scientific, practical and economic results. Experiences have shown that dosing the antiviral agent and choosing an optimal size of meristematic inoculums is particularly important in achieving satisfactory levels of regeneration, the optimal combination being 0.2 to 0.4 mm and 30 mg / L Ribavirin in a significant reduction of PPV content of viral particles.
- Subjects
ROMANIA; PLUM diseases &; pests; PATHOGENIC microorganisms; MOLECULAR diagnosis; STANDARDIZATION; FRUIT diseases &; pests; PREVENTION
- Publication
Annals of the Romanian Society for Cell Biology, 2011, Vol 16, Issue 1, p292
- ISSN
2067-3019
- Publication type
Article