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- Title
DNA, Protein and Lipid Peroxidation as Oxidative Stress Biomarkers in Seminal Plasma of Smoker and Nonsmoker Infertile Men.
- Authors
Ali Jorsaraei, Seyed Gholam; Mahjoub, Soleiman; Gholinezhad-Chari, Maryam; Pasha, Yousefreza Yousefnia; Tahmasbpour, Eisa; Shafi, Hamid
- Abstract
Objective: Cigarette smoke includes number of substances that may induce oxidative damage and as the results cause poor sperm quality in male subjects. Materials and methods: Semen sample provided from normozoospermic nonsmoker (n=26), infertile smoker (n=29) and infertile nonsmoker men (n=31). Semen analysis was performed manually according to the world health organization (WHO) standards guidelines. Malondialdehyde (MDA), as a biomarker of lipid peroxidation, was measured by Thiobarbituric acid reaction (TBAR) method. Protein carbonyl (PC) content, as a biomarker of protein oxidation, was assayed using spectrophotometry technique. 8-OHdG level, as a biomarker of DNA oxidation, was also measured using ELISA kit. Data were analyzed by SPSS software and the p<0.05 was considered as significant. Results: Normozoospermic subjects showed a significant higher sperm quality (sperm counts, sperm motility and sperm normal morphology) compared with infertile groups (p<0.001). A trend for higher sperm quality, particularly total sperm counts (p=0.09) and motility (p=0.12), was found for infertile nonsmokers compared with infertile smokers. The mean of MDA concentration in normozoospermic nonsmoker subjects (0.60 ± 0.18 nmol/ml) was significantly lower than the infertile smoker men (0.73 ± 0.19 nmol/ml) (p<0.05). We didn't found a significant difference regarding the seminal PC contents between three groups; however, the mean of PC concentration in normozoospermic nonsmoker subjects (4.66 ± 1.73 nmol/mg) was slightly lower than the infertile groups. The mean value of 8-OHdG in normozoospermic nonsmoker subjects (286.38 ± 121.96 ng/ml) was significantly lower than in the both infertile groups (p<0.001). A higher concentration of seminal 8-OHdG was found in infertile smokers compared with infertile nonsmokers (p<0.05). Conclusion: The negative effect of cigarette smoking is positively related to decreased sperm parameter quality. This is may be due to cigarette toxic components that cause to increase of seminal free radicals and as the result sperm membrane lipids peroxidation, proteins and DNA oxidation, and eventually low sperm quality in smoker men.
- Subjects
CIGARETTE smokers; MALE infertility; LIPID peroxidation (Biology); OXIDATIVE stress; BIOMARKERS; SEMINAL proteins; SEMEN analysis
- Publication
Caspian Journal of Applied Sciences Research, 2015, Vol 4, Issue 9, p16
- ISSN
2251-9114
- Publication type
Article