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- Title
The role of CD8<sup>+</sup> cells in the establishment and maintenance of aTrichuris murisinfection.
- Authors
Humphreys, N. E.; Worthington, J. J.; Little, M. C.; Rice, E. J.; Grencis, R. K.
- Abstract
Chronic infection by the caecal-dwelling intestinal murine nematode Trichuris muris occurs if given as a high-dose infection to ‘susceptible’ AKR mice or as a low-dose infection to the normally ’resistant‘ C57BL16 mouse strain. Both regimes result in a type 1 cytokine response, i.e. high levels of IFN-γ and IL-12. Here we show this susceptible response is associated with a large population of CD8+ IFN-γ+ cells within the mesenteric lymph nodes and numerous CD8+ cells infiltrating the caecal mucosa. Despite this, the in vivo abolition of CD8+ cells within AKR and C57BL16 mice, either prior to infection or once infection has become established, failed to affect chronicity, implying that CD8+ T cells are not essential for the initiation or maintenance of the susceptible response to T. muris. Interestingly, the percentage of IFN-γ+ CD4+ cells increased in treated groups, perhaps in a compensatory role. The majority of antigen-specific cytokine responses were comparable in both treated and control groups, although IL-5 was fivefold higher in animals receiving anti-CD8 mAbs and IFN-γ was also raised in treated mice. Mastocytosis was unaltered by CD8 depletion, however, paradoxically, eosinophilia within the caecum was reduced in treated mice. Together these data clearly demonstrate that CD8+ T cells are associated with chronic T. muris infection; however, these cells are dispensable for both the early and late phases of this response, but do appear to play a role in the regulation of certain cytokines and caecal eosinophilia.
- Subjects
CD antigens; HELMINTHS; TRICHURIASIS; PARASITES; IMMUNOLOGY
- Publication
Parasite Immunology, 2004, Vol 26, Issue 4, p187
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1111/j.0141-9838.2004.00702.x