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- Title
Biological and Radioimmunological Evidence for Melanocyte Stimulating Hormones (MSH) of Extrapituitary Origin in the Rat Brain.
- Authors
Vaudry, H.; Tonon, M.C.; Delarue, C.; Vaillant, R.; Kraicer, J.
- Abstract
The possible existence of extrapituitary melanocyte stimulating hormone (MSH) in various regions of the rat brain has been studied in intact and hypophysectomized rats. Using a sensitive and specific radioimmunoassay (RIA), αMSH has been found in a number of brain regions in intact rats. The standard curves of synthetic αMSH and the dilution curves for pars intermedia nervosa (PIN), pars distalis (PD), hypothalamus and thalamus extracts were strictly parallel. The αMSH concentrations were measured in PIN (6,225 ± 962 ng/mg wet tissue); PD (12.5 ± 1.41 ng/mg); pineal (380 ± 29 ng/g wet tissue); hypothalamus (645 ± 161 ng/g) and thalamus (33.3 ± 5.26 ng/g). In rats hypophysectomized for 1 or 2 months, the highest concentrations of immunoreactive αMSH were found in pineal (353 ± 140 ng/g wet tissue), hypothalamus (85.8 ±14.1 ng/g) and thalamus (39.8 ± 13.9 ng/g). Hypophysectomy significantly reduced hypothalamic MSH content and concentration but did not alter MSH concentration in pineal and thalamus. From these results, we conclude that hypothalamic αMSH is, in part, of hypophyseal origin while pineal and thalamus «MSH does not originate from the pituitary. After Sephadex G-25 gel filtration, synthetic αMSH and PIN extracts showed a single peak of both bioactive and immunoreactive αMSH. In the same conditions, extracts from the 5 brain regions studied in hypophysectomized rats chromatographed as a single peak of immunoreactive MSH but as 2 peaks of apparent bioactive MSH, 1 concident with synthetic αMSH and the other far after the salt volume. We conclude that αMSH is found in a number of brain areas and its presence after hypophysectomy would indicate synthesis within the central nervous system. Copyright © 1978 S. Karger AG, Basel
- Publication
Neuroendocrinology, 1978, Vol 27, Issue 1/2, p9
- ISSN
0028-3835
- Publication type
Article
- DOI
10.1159/000122796