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- Title
Serological investigation of vector-borne disease in dogs from rural areas of China
- Authors
Wang, Shiwen; He, Jing; Zhang, Lijuan
- Abstract
Abstract: Objective: To evaluate the Anaplasma phagocytophilum (A. phagocytophilum), Ehrlichia canis (E. canis), Dirofilaria immitis (D. immitis) (canine heartworm), Borrelia burgdorferi (B. burgdorferi) infections in countryside dogs from Yunnan, Hainan and Anhui provinces. Methods: Serum samples were collected from 26 dogs in Yunnan, Hainan and Anhui provinces. The samples were tested using a commercial ELISA rapid diagnostic assay kit (SNAP® 4Dx®; IDEXX Laboratories, Inc. U.S.A.). Meanwhile, indirect immunofluorescence assay (IFA) recommended by WHO was conducted to detect IgG to A. phagocytophilum. Two methods were analyzed and compared. Results: The number of serologically positive dogs for IgG to A. phagocytophilum was only 2 which was from Hainan province and none of the 26 dogs responded positive for E. canis, D. immitis (canine heartworm), and B. burgdorferi by ELISA rapid diagnostic method. The number of serologically positive dogs for IgG to A. phagocytophilum was 13 (50%) by IFA method. Data of the two methods were analyzed by statistical software and the difference was statistically significant (P=0.002). Conclusions: It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method. However, we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including A. phagocytophilum, E. canis, D. immitis (canine heartworm), and B. burgdorferi which have public health significance.
- Subjects
YUNNAN Sheng (China); HAINAN Sheng (China); ANHUI Sheng (China); CHINA; DOGS; DIROFILARIA immitis; BORRELIA burgdorferi; ENZYME-linked immunosorbent assay; EPIDEMIOLOGY
- Publication
Asian Pacific Journal of Tropical Biomedicine, 2012, Vol 2, Issue 2, p102
- ISSN
2221-1691
- Publication type
Article
- DOI
10.1016/S2221-1691(11)60201-3