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- Title
EMSCs‐Seeded Micro‐Stripe Patterned Polycaprolactone Promoting Sciatic Nerve Regeneration.
- Authors
Lu, Naiyan; Wang, Xinhe; Li, Xu; Shi, Wentao; Wang, Xun; Zou, Yin; Yang, Guofeng; Tang, Xue; Zhang, Zhijian; Xiang, Wen; Weng, Yuyan
- Abstract
Transplantation of a synthetic polymer nerve conduit carrying stem cells is promising for treating peripheral nerve injury. In this study, a micropatterned inner surface of polycaprolactone (PCL) nerve conduit cellularized with nasal respiratory mucosa‐derived ectoderm mesenchymal stem cells (EMSCs) is adopted for peripheral nerve regeneration. The PCL microstructure grating is fabricated by soft nanoimprint lithography (NIL), whose templates can be easily transferred from the Ebeam photoresist pattern. The most suitable microstructure is carefully chosen to support EMSCs alignment and nerve regeneration. In vitro, the micropatterned PCL nerve conduit greatly enhanced the directional alignment of EMSCs and the MBP and S100 expression of myelin proteins. Additionally, the micropatterned conduit preseeded with EMSCs significantly stimulated the axon‐directed extension of neural stem cells. In vivo, micropatterned PCL conduits cotransplanted with EMSCs enhanced motor function and promoted sciatic nerve regeneration and extension in a rat model of sciatic nerve defect. Taken together, this "all‐in‐one" nerve guidance conduit makes sciatic nerve regeneration and morphological repairing accelerated, which has a potential clinical application in neural tissue engineering.
- Subjects
SCIATIC nerve; NERVOUS system regeneration; POLYCAPROLACTONE; PERIPHERAL nervous system; PERIPHERAL nerve injuries; MYELIN proteins; NEURAL stem cells
- Publication
Advanced Materials Interfaces, 2023, Vol 10, Issue 5, p1
- ISSN
2196-7350
- Publication type
Article
- DOI
10.1002/admi.202201929