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- Title
Synthetic Nucleosomes Reveal that GlcNAcylation Modulates Direct Interaction with the FACT Complex.
- Authors
Raj, Ritu; Lercher, Lukas; Mohammed, Shabaz; Davis, Benjamin G.
- Abstract
Transcriptional regulation can be established by various post-translational modifications (PTMs) on histone proteins in the nucleosome and by nucleobase modifications on chromosomal DNA. Functional consequences of histone O-GlcNAcylation (O-GlcNAc=O-linked β-N-acetylglucosamine) are largely unexplored. Herein, we generate homogeneously GlcNAcylated histones and nucleosomes by chemical post-translational modification. Mass-spectrometry-based quantitative interaction proteomics reveals a direct interaction between GlcNAcylated nucleosomes and the 'facilitates chromatin transcription' (FACT) complex. Preferential binding of FACT to GlcNAcylated nucleosomes may point towards O-GlcNAcylation as one of the triggers for FACT-driven transcriptional control.
- Subjects
CHROMATIN; ACYLATION; HISTONES; BASE pairs; PROTEOMICS
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 31, p8918
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201603106