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- Title
NMR Spectroscopy of Soluble Protein Complexes at One Mega-Dalton and Beyond.
- Authors
Mainz, Andi; Religa, Tomasz L.; Sprangers, Remco; Linser , Rasmus; Kay, Lewis E.; Reif, Bernd
- Abstract
Bigger is better: Sequential backbone assignments are obtained by NMR spectroscopy for a 1 MDa proteasome complex. The method relies on immobilization of a soluble protein complex by magic‐angle spinning. Deuteration and proton detection of exchangeable sites and paramagnetic relaxation enhancement enables exploration of structural and dynamic properties of supramolecular assemblies at atomic resolution.
- Subjects
PROTEIN synthesis; PROTEIN structure; NUCLEAR magnetic resonance spectroscopy; ROTATIONAL diffusion; VISCOSITY solutions; PROTEASOME regulation; BIOCHEMICAL substrates
- Publication
Angewandte Chemie International Edition, 2013, Vol 52, Issue 33, p8746
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201301215