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- Title
Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation.
- Authors
Jinek, Martin; Fabian, Marc R.; Coyle, Scott M.; Sonenberg, Nahum; Doudna, Jennifer A.
- Abstract
GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.
- Subjects
RNA; CRYSTALS; MESSENGER RNA; GENE silencing; PROTEIN binding
- Publication
Nature Structural & Molecular Biology, 2010, Vol 17, Issue 2, p238
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.1768