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- Title
A Single Residue Determines the Cooperative Binding Property of a Primosomal DNA Replication Protein, PriB, to Single-Stranded DNA.
- Authors
Yen-Hua Huang; Hsin-Hsien Lin; Cheng-Yang Huang
- Abstract
The article presents a study which characterizes and compares the single-stranded DNA (ssDNA)-binding properties of a primosomal DNA replication protein (PriB) Salmonella enterica. The study employs gel filtration, gel shifts, and glutaraldehyde cross-linking. Results proposed the binding modes for forming a stable complex of PriB with ssDNA of 25 nucleotides (nt).
- Subjects
SINGLE-stranded DNA binding proteins; DNA replication; SALMONELLA enterica; GEL permeation chromatography; GLUTARALDEHYDE; NUCLEOTIDES
- Publication
Bioscience, Biotechnology & Biochemistry, 2012, Vol 76, Issue 6, p1110
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.110938