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- Title
Cloning, Expression, and Identification of Immunological Activity of an Allergenic Protein in Tartary Buckwheat.
- Authors
Zhuanhua Wang; Lan Wang; Wenjun Chang; Yuying Li; Zheng Zhang; Wieslander, Gunilla; Norback, Dan
- Abstract
The article presents a study that examines cloning, expression and identification of immunological activity of an allergenic protein in tartary buckwheat. The cDNA fragment encoding allergenic protein in tartary buckwheat was obtained using reverse transcription polymerase chain reaction. The cDNA cloned contained 768 nucleotides which include 588 nucleotides in the open reading frame (ORF) and 180 nucleotides in the terminal sequence. An analysis of immunological activity showed that the recombinant protein had a specific binding activity.
- Subjects
MOLECULAR cloning; CLONING; MOLECULAR genetics; GENE expression; BUCKWHEAT; POLYMERASE chain reaction; NUCLEOTIDES; NUCLEIC acids
- Publication
Bioscience, Biotechnology & Biochemistry, 2006, Vol 70, Issue 5, p1195
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.70.1195