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- Title
Streptomyces tunisiensis sp. nov., a novel Streptomyces species with antibacterial activity.
- Authors
Slama, Nedra; Mankai, Houda; Ayed, Ameni; Mezhoud, Karim; Rauch, Catherine; Lazim, Hadeer; Barkallah, Insaf; Gtari, Maher; Limam, Ferid
- Abstract
A novel actinomycete strain designated CN-207 was isolated from northern Tunisian soil. This strain exhibited potent broad spectrum antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several other Gram-positive and Gram-negative bacteria. Strain CN-207 developed greyish aerial mycelium and pale grey substrate mycelium on yeast extract/malt agar. The isolate produced branching vegetative mycelia with sporangiophores bearing sporangia developing at a late stage of growth. The sporangia contained smooth, non-motile spores. Chemotaxonomic characteristics of strain CN-207 were typical of the Streptomyces genus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CN-207 belonged to the genus Streptomyces, and was most closely related to Streptomyces griseoincarnatus DSM 40274, Streptomyces variabilis DSM 40179, Streptomyces labedae DSM 41446 and Streptomyces erythrogriseus DSM 40116. Low DNA-DNA relatedness values were recorded between strain CN-207 and its closest phylogenetic neighbours. Strain CN-207 was also distinguished from the nearest phylogenetic neighbours using a combination of morphological and phenotypic characteristics. On the basis of its phenotypic and molecular properties, strain CN-207 is considered as a novel species of the Streptomyces genus, for which the name Streptomyces tunisiensis sp. nov. is proposed. The type strain is CN-207 (=JCM 17589 = DSM 42037).
- Subjects
REPRODUCTIVE isolation; ISOLATION of biotechnological microorganisms; STREPTOMYCES; ANTIBACTERIAL agents; GRAM-positive bacteria; GRAM-negative bacteria; BACTERIAL growth; CHEMOTAXONOMY
- Publication
Antonie van Leeuwenhoek, 2014, Vol 105, Issue 2, p377
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-013-0086-z