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- Title
Absence of repellents in Ustilago maydis induces genes encoding small secreted proteins.
- Authors
Teertstra, Wieke R.; Krijgsheld, Pauline; Wösten, Han A. B.
- Abstract
The rep1 gene of the maize pathogen Ustilago maydis encodes a pre-pro-protein that is processed in the secretory pathway into 11 peptides. These so-called repellents form amphipathic amyloid fibrils at the surface of aerial hyphae. A SG200 strain in which the rep1 gene is inactivated (∆ rep1 strain) is affected in aerial hyphae formation. We here assessed changes in global gene expression as a consequence of the inactivation of the rep1 gene. Microarray analysis revealed that only 31 genes in the ∆ rep1 SG200 strain had a fold change in expression of ≥2. Twenty-two of these genes were up-regulated and half of them encode small secreted proteins (SSPs) with unknown functions. Seven of the SSP genes and two other genes that are over-expressed in the ∆ rep1 SG200 strain encode proteins that can be classified as secreted cysteine-rich proteins (SCRPs). Interestingly, most of the SCRPs are predicted to form amyloids. The SCRP gene um00792 showed the highest up-regulation in the ∆ rep1 strain. Using GFP as a reporter, it was shown that this gene is over-expressed in the layer of hyphae at the medium-air interface. Taken together, it is concluded that inactivation of rep1 hardly affects the expression profile of U. maydis, despite the fact that the mutant strain has a strong reduced ability to form aerial hyphae.
- Subjects
GENES; USTILAGO maydis; CORN; PATHOGENIC microorganisms; AMYLOID beta-protein; HYPHAENE; PROTEINS; CYSTEINE proteinases
- Publication
Antonie van Leeuwenhoek, 2011, Vol 100, Issue 2, p219
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-011-9581-2