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- Title
Expression of MMP-10, MMP-21, MMP-26, and MMP-28 in Merkel cell carcinoma.
- Authors
Suomela, Sari; Koljonen, Virve; Skoog, Tiina; Kukko, Heli; Böhling, Tom; Saarialho-Kere, Ulpu
- Abstract
Merkel cell carcinoma (MCC) is an aggressive cutaneous tumor with poor outcome and increasing incidence. We examined by immunohistochemistry the expression of three novel matrix metalloproteinases (MMPs)-MMP-21, MMP-26, and MMP-28-in 44 primary MCC tumors and six lymph node metastases while MMP-10 served as a positive control. Their mRNA expression was also studied in the UISO MCC cell line basally and after various stimulations using quantitative real-time PCR. MMP-28 was observed in tumor cells of 15/44 samples especially in tumors <2 cm in diameter (p = 0.015) while 21/44 specimens showed MMP-28 in the tumor stroma. Expression of MMP-21 was demonstrated in tumor cells of 13/43 samples. MMP-26, instead, was positive in stromal cells (17/44) and its expression associated with tumors >or=2 cm in diameter (p = 0.006). Stromal expression of MMP-10 was the most frequent finding of the studied samples (31/44), but MMP-10 was detected also in tumor cells (17/44). Most of the metastatic lymph nodes expressed MMP-10 and MMP-26. MMP-10, MMP-21, and MMP-28 mRNAs were basally expressed by the UISO cells, and the corresponding proteins were detectable by immunostaining of cultured cells. IFN-alpha and TNF-alpha downregulated MMP-21 and MMP-28 expression. Our results suggest that novel MMPs may have a role in MCC pathogenesis: especially that MMP-26 expression in stroma is associated with larger tumors with poor prognosis. Expression of MMP-21 and MMP-28 seems to associate with the tumors of lesser malignant potential. We also confirm the previous finding on the role of MMP-10 in MCC pathogenesis.
- Subjects
CELL lines; COMPARATIVE studies; RESEARCH methodology; MEDICAL cooperation; METASTASIS; NEUROENDOCRINE tumors; PROTEOLYTIC enzymes; RESEARCH; SKIN tumors; EVALUATION research; MATRIX metalloproteinases
- Publication
Virchows Archiv: European Journal of Pathology, 2009, Vol 455, Issue 6, p495
- ISSN
0945-6317
- Publication type
journal article
- DOI
10.1007/s00428-009-0856-1