We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
cGMP regulates hydrogen peroxide accumulation in calcium-dependent salt resistance pathway in Arabidopsis thaliana roots.
- Authors
Li, Jisheng; Wang, Xiaomin; Zhang, Yanli; Jia, Honglei; Bi, Yurong
- Abstract
3′,5′-cyclic guanosine monophosphate (cGMP) is an important second messenger in plants. In the present study, roles of cGMP in salt resistance in Arabidopsis roots were investigated. Arabidopsis roots were sensitive to 100 mM NaCl treatment, displaying a great increase in electrolyte leakage and Na/K ratio and a decrease in gene expression of the plasma membrane (PM) H-ATPase. However, application of exogenous 8Br-cGMP (an analog of cGMP), HO or CaCl alleviated the NaCl-induced injury by maintaining a lower Na/K ratio and increasing the PM H-ATPase gene expression. In addition, the inhibition of root elongation and seed germination under salt stress was removed by 8Br-cGMP. Further study indicated that 8Br-cGMP-induced higher NADPH levels for PM NADPH oxidase to generate HO by regulating glucose-6-phosphate dehydrogenase (G6PDH) activity. The effect of 8Br-cGMP and HO on ionic homeostasis was abolished when Ca was eliminated by glycol-bis-(2-amino ethyl ether)- N,N,N′,N′-tetraacetic acid (EGTA, a Ca chelator) in Arabidopsis roots under salt stress. Taken together, cGMP could regulate HO accumulation in salt stress, and Ca was necessary in the cGMP-mediated signaling pathway. HO, as the downstream component of cGMP signaling pathway, stimulated PM H-ATPase gene expression. Thus, ion homeostasis was modulated for salt tolerance.
- Subjects
CYCLIC guanylic acid; PLANT plasma membranes; ARABIDOPSIS thaliana; HYDROGEN peroxide; HOMEOSTASIS; GERMINATION; GLUCOSE-6-phosphate dehydrogenase; PLANT roots
- Publication
Planta: An International Journal of Plant Biology, 2011, Vol 234, Issue 4, p709
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-011-1439-3