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- Title
YAP regulates porcine skin-derived stem cells self-renewal partly by repressing Wnt/β-catenin signaling pathway.
- Authors
Yan, Hong-Chen; Sun, Yu; Zhang, Ming-Yu; Zhang, Shu-Er; Sun, Jia-Dong; Dyce, Paul W.; Klinger, Francesca Gioia; De Felici, Massimo; Shen, Wei; Cheng, Shun-Feng
- Abstract
Skin-derived stem cells (SDSCs) are a class of adult stem cells (ASCs) that have the ability to self-renew and differentiate. The regulation mechanisms involved in the differentiation of SDSCs are a hot topic. In this paper, we explore the link between the transcriptional regulator yes-associated protein (YAP) and the fate of porcine SDSCs (pSDSCs). We found that lysophosphatidylcholine (LPC) activates YAP, promotes pSDSCs pluripotency, and counteracts transdifferentiation of pSDSCs into porcine primordial germ cell-like cells (pPGCLCs). YAP promotes the pluripotent state of pSDSCs by maintaining the high expression of the pluripotency genes Oct4 and Sox2. The overexpression of YAP prevented the differentiation of pSDSCs, and the depletion of YAP by small interfering RNA (siRNAs) suppressed the self-renewal of pSDSCs. In addition, we found that YAP regulates the fate of pSDSCs through a mechanism related to the Wnt/β-catenin signaling pathway. When an activator of the Wnt/β-catenin signaling pathway, CHIR99021, was added to pSDSCs overexpressing YAP, the ability of pSDSCs to differentiate was partially restored. Conversely, when XAV939, an inhibitor of the Wnt/β-catenin signaling pathway, was added to YAP knockdown pSDSCs a higher self-renewal ability resulted. Taken together, our results suggested that YAP and the Wnt/β-catenin signaling pathway interact to regulate the fate of pSDSCs.
- Subjects
CELLULAR signal transduction; STEM cells; SMALL interfering RNA; WNT proteins; GERM cells; WNT signal transduction; CATENINS; CELL differentiation; SKIN; ANIMAL experimentation; SWINE; RNA; GENE expression; PHOSPHOLIPIDS
- Publication
Histochemistry & Cell Biology, 2022, Vol 157, Issue 1, p39
- ISSN
0948-6143
- Publication type
Article
- DOI
10.1007/s00418-021-02034-4