We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Fine specificities of two lectins from Cymbosema roseum seeds: a lectin specific for high-mannose oligosaccharides and a lectin specific for blood group H type II trisaccharide.
- Authors
Dam, Tarun K; Cavada, Benildo S; Nagano, Celso S; Rocha, Bruno AM; Benevides, Raquel G; Nascimento, Kyria S; de Sousa, Luiz AG; Oscarson, Stefan; Brewer, C Fred
- Abstract
The legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the “core” trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fucα1,2Galα1-4GlcNAc-). Thermodynamic and hemagglutination inhibition studies reveal the fine binding specificities of the two lectins. Data obtained with a complete set of monodeoxy analogs of the core trimannoside indicate that CRL I recognizes the 3-, 4- and 6-hydroxyl groups of the α(1,6) Man residue, the 3- and 4-hydroxyl group of the α(1,3) Man residue and the 2- and 4-hydroxyl groups of the central Man residue of the trimannoside. CRL I possesses enhanced affinities for the Man5 oligomannose glycan and a biantennary complex glycan as well as glycoproteins containing high-mannose glycans. On the other hand, CRL II distinguishes the blood group H type II epitope from the Lewisx, Lewisy, Lewisa and Lewisb epitopes. CRL II also distinguishes between blood group H type II and type I trisaccharides. CRL I and CRL II, respectively, possess differences in fine specificities when compared with other reported mannose and fucose recognizing lectins. This is the first report of a mannose-specific lectin (CRL I) and a blood group H type II-specific lectin (CRL II) from seeds of a member of the Diocleinae subtribe.
- Subjects
LEGUMES; LECTINS; MANNOSE; OLIGOSACCHARIDES; SACCHARIDES; THERMODYNAMICS; BLOOD agglutination
- Publication
Glycobiology, 2011, Vol 21, Issue 7, p925
- ISSN
0959-6658
- Publication type
Article
- DOI
10.1093/glycob/cwr025